Purpose
For the detection and counting of Pseudomonas aeruginosa - membrane filtration method
Inspection principle
Gelatin peptone and acid-hydrolyzed casein provide nitrogen sources; Glycerol provides a carbon source; Potassium sulfate and magnesium chloride can promote the production of pseudomonas pigment. AGAR is the coagulant of the culture medium. Naphthidine acid inhibits Gram-negative bacilli that are not Pseudomonas.
Usage
Weigh 52.4 grams of this product, add it to 900mL of distilled water, heat and boil to dissolve, aliquot, sterilize at 121℃ under high pressure for 15 minutes. Before use, add the accompanying reagents antibiotic solution (04-059) and glycerin solution (04-060) in a sterile environment (for usage and dosage, please refer to the instructions of the accompanying reagents). Mix well, aliquot and set aside.
Quality control
Prepare the culture medium according to the label usage, inoculate the following quality control strains, and place it in aerobic culture at 36±1℃ for 24-48 hours.